SUSCEPTIBILITY TO ANTISEPTICS IN BACTERIAL FLORA OF WRESTLERS' SKIN

The purpose of the research was to study the bacteriocenosis of intact skin in wrestlers and to determine the susceptibility to antiseptic preparations in bacterial flora.

Methods and organization of the research. 15 youth wrestlers took part in the experiment. All wrestlers were engaged in national wrestling and belt wrestling, qualifications ranged from the 1st Adult Category to the Master of Sports. For microbiological analysis, the washings were made with sterile cotton swab before and after training from intact skin of medial part of forearms of wrestlers with an area of 10 cm2. Then, this swab was placed in a test tube with 1 ml of sterile Amies transport medium without charcoal, transported within 24 hours to the laboratory, where it was sown on selective media by the method of sector crops: 5% blood agar and egg-yolk salt agar. After 48 hours, the number of grown microorganisms in the sectors was determined. Isolated colonies of microorganisms were identified using the MALDI Microflex Biotyper mass spectrometer (Bruker, Germany) in the laboratory of the Interdisciplinary Center for Collective Use of the Kazan Federal University. The quality of bacteriocenosis was assessed according to the dominance index, the formula of which was proposed by F.D. Mordukhai-Boltovskaya. The sensitivity of all isolated bacteria to antiseptics («Veltosept-2», «Kutasept», 0.05% aqueous solution of Chlorhexidine bigluconate, «Miramistine», 3% Hydrogen peroxide solution) was determined by the method described in the federal clinical guidelines (2014).

Results and discussion. As a result, it was found that Staphylococcus aureus became the dominant species of skin microbiocenosis after training, and Bacillus cereus was present as the dominant species both before and after training. This may indicate skin dysbiosis, which leads to the development of skin infectious diseases. As a result of the research, it was determined that the skin antiseptic «Veltosept-2» is the drug of choice for the prevention and treatment of infectious skin diseases in wrestlers, the etiological pathogen of which can be almost all dominant, subdominant and other bacteria isolated after training from the skin of wrestlers' forearms (B. cereus, S. aureus, A. Iwoffii, S.saprophyticus, S. epidermidis, M.luteus).

Conclusion. Athletes of contact sports demonstrate an increased risk of developing skin infectious diseases and require effective means of protection and prevention. 

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