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Vol 11 (4) 2023

Article

POLYMORPHISM OF THE SEROTONIN RECEPTOR 5-HTR2A GENE AND METABOLOMIC PROFILE IN THE YAKUT STUDENTS-BOXERS (0.79 Mb, pdf) Read
Authors:
Solov'eva Natal'ya Alekseevna
Kolosova Ol'ga Nikolaevna
Zasimova Ekaterina Zaharovna
Kychkina Oksana Innokent'evna
Slepcov Igor' Vital'evich
Dmitriev Nikolay Alexandrovich
Keywords:
boxers
Yakuts
serotonin
gene
metabomics
blood plasma
GC-MS
Annotation:

The purpose of the research is to analyze the features of the frequency distribution of alleles and genotypes of polymorphism of the serotonin 5–HTR2A receptor gene (rs 6311) and the metabolomic profile in students of the Yakut population engaged in boxing.

Methods and organization of research. In total, 123 male students of various faculties of the M.K. Ammosov NEFU, Yakut ethnicity, aged 19-25 years, 20 of them are students of specialized groups of the Institute of Physical Culture and Sports the M.K. Ammosov NEFU engaged in boxing, took part in the study. Participation in the study was voluntary and was accompanied by the signing of informed consent. The analysis of the frequency distribution of the polymorphic variant of the serotonin 5-HTR2A receptor gene (rs 6311) and the metabolomic profile in Yakut students-boxers was carried out for the first time. The laboratory part of the study, including molecular genetic studies by polymerase chain reaction (PCR) methods/restriction fragment length polymorphism (RFLP) and the analysis of the metabolomic profile, was carried out on the basis of the Department of Environmental and Medical Biochemistry, Biotechnology of the Institute of Biological Problems of the Cryolithozone SB RAS.

The research results and their discussion. As a result of the studies, it was found that the studied group of students-boxers was characterized by a predominance of the homozygous genotype GG (60%) and the allele G (0.816) and the absence of a homozygous genotype for allele A. The analysis of the features of the metabolomic profile of the blood plasma of students-boxers depending on the genotypes demonstrated significant differences in the concentration of some fatty, organic and inorganic acids, sugars and their derivatives.

Conclusion. Considering the results obtained on the predominance of carriers in the Yakut population as a whole of the dominant G allele, which, according to the literature, is associated with a quick assessment of the visual stimulus as negative, it can be assumed that this biological marker is one of the evolutionary adaptation factors that determine the functioning of the central nervous system, allowing survival in extreme climatic and geographical conditions of the North. The absence in the group of Yakut students engaged in boxing of a recessive homozygous genotype for the A allele, associated with the involvement of significantly greater brain resources, compared to carriers of the dominant G allele, for evaluating a visual stimulus as negative can be considered as a factor shaping the ability to make a quick balanced assessment "dangerous" situation for an athlete in the ring and a biological marker for selection of candidates for specialized boxing groups. The revealed differences in the metabolomic profiles of blood plasma in the groups of boxers, depending on the genotypes, are due to the different course of homeostatic processes.

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